Biocompatibility of three new calcium silicate‐based endodontic sealers on human periodontal ligament stem cells. (22nd October 2016)

• Record Type: Journal Article
• Authors: Collado‐González, M.; García‐Bernal, D.; Oñate‐Sánchez, R. E.; Ortolani‐Seltenerich, P. S.; Lozano, A.; Forner, L.; Llena, C.; Rodríguez‐Lozano, F. J.
• Is Part Of: International endontic journal. Volume 50:Number 9(2017:Sep.)
• Page Start: 875
• Page End: 884
• Publication Date: 2016-10-22
• Subjects: calcium silicate‐based endodontic sealers -- cytotoxicity -- human periodontal ligament stem cells -- mineral trioxide aggregate; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12703
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To evaluate the biocompatibility of three calcium silicate‐based endodontic sealers, Bioroot BC Sealer (Septodont, Saint‐Maur‐des‐Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano‐ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells ( h PDLSCs). Methodology: Human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound‐healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, h PDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One‐way analysis of variance (anova ) followed by a Bonferroni post‐test was performed ( P < 0.05). Results: At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer (BR) and Nano‐ceramic Sealer (NCS), but not Endoseal MTA (ES). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation ( P < 0.05). Similar results were obtained in a cell death assay. In addition, h PDLSCs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR. Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs. Conclusions: BR and NCS were associated with better cytocompatibility than ES. Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate‐based endodontic sealers for clinical application.