MiR‐429 suppresses glioblastoma multiforme by targeting SOX2. (27th July 2017)

• Record Type: Journal Article
• Authors: Dong, Huixiao; Hao, Xiuzhen; Cui, Benliang; Guo, Meiling
• Is Part Of: Cell biochemistry and function. Volume 35:Number 5(2017)
• Page Start: 260
• Page End: 268
• Publication Date: 2017-07-27
• Subjects: glioblastoma multiforme -- miR‐429 -- SOX2; Cytochemistry -- Periodicals; Cell metabolism -- Periodicals; Biochemistry -- Periodicals; Cytology -- Periodicals; 572
• DOI: 10.1002/cbf.3271
• Languages: English
• ISSNs: 0263-6484

Abstract

Abstract : Accumulating evidence has shown that miR‐429 plays an important role in the development and progression of tumour. However, the role of miR‐429 in glioblastoma multiforme (GBM) remains largely unknown. The present study is designed to investigate the function of miR‐429 in GBM and to explore the molecular mechanism underlying its function. The expression level of miR‐429 was detected in GBM tissues and cell lines by quantitative real‐time polymerase chain reaction. The effect of overexpression of miR‐429 on in vitro cell proliferation, apoptosis and invasion was examined. Western blot analysis was used to detect the influence of miR‐429 on the expression of target gene, and Pearson analysis was used to calculate the correlation between the expression of targets gene and the miR‐429 in GBM tissues. Our study shows that miR‐429 is downregulated in GBM tissues compared with noncancerous tissues ( P < .01). In addition, the expression of miR‐429 in GBM cell lines is also significantly lower ( P < .01). Enforced expression of miR‐429 inhibits GBM cells proliferation, induces apoptosis and suppresses invasion and leads to the downregulation of the SOX2 protein. Moreover, the expression level of miR‐429 in GBM tissues shows inverse relationship with the expression level of SOX2 protein. Our findings suggest that miR‐429 represents a potential tumour‐suppressive miRNA and plays an important role in GBM progression by directly targeting SOX2. Abstract : The present study is designed to investigate the function of miR‐429 in glioblastoma multiforme (GBM) and to explore the molecular mechanism underlying its function. Our study shows that miR‐429 is downregulated in GBM tissues. Enforced expression of miR‐429 inhibits GBM cells proliferation, induces apoptosis and suppresses invasion and leads to the downregulation of the SOX2 protein. Moreover, our findings suggest that miR‐429 represents a potential tumour‐suppressive miRNA and plays an important role in GBM progression by directly targeting SOX2.