High-throughput system-wide engineering and screening for microbial biotechnology. (August 2017)

• Record Type: Journal Article
• Authors: Vervoort, Yannick; Linares, Alicia Gutiérrez; Roncoroni, Miguel; Liu, Chengxun; Steensels, Jan; Verstrepen, Kevin J
• Is Part Of: Current opinion in biotechnology. Volume 46(2017)
• Page Start: 120
• Page End: 125
• Publication Date: 2017-08
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2017.02.011
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: MAGE and CRISPR/Cas systems are increasingly used for large-scale genome engineering. Population based omics tools are optimized for higher throughputs but lack single cell resolution. Nanoliter-scale capsule or droplet reactors can be used for high-throughput single cell screening. Microchips allow ultra-high throughput picoliter-scale single cell phenotyping. Abstract : Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their experiments to the nanoliter and picoliter scale and the single-cell level. In this review, we summarize different high-throughput system-wide genome engineering and screening strategies for microbes. More specifically, we will emphasize the use of multiplex automated genome evolution (MAGE) and CRISPR/Cas systems for high-throughput genome engineering and the application of (lab-on-chip) nanoreactors for high-throughput single-cell or population screening.