Biodegradation of hydrocarbon mixtures in surface waters at environmentally relevant levels – Effect of inoculum origin on kinetics and sequence of degradation. (October 2017)

• Record Type: Journal Article
• Authors: Birch, Heidi; Hammershøj, Rikke; Comber, Mike; Mayer, Philipp
• Is Part Of: Chemosphere. Volume 184(2017)
• Page Start: 400
• Page End: 407
• Publication Date: 2017-10
• Subjects: Biodegradation kinetics -- Volatile petroleum hydrocarbons -- Composed mixtures -- SPME -- Passive dosing; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.05.169
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Biodegradation is a dominant removal process for many organic pollutants, and biodegradation tests serve as tools for assessing their environmental fate within regulatory risk assessment. In simulation tests, the inoculum is not standardized, varying in microbial quantity and quality, thereby potentially impacting the observed biodegradation kinetics. In this study we investigated the effect of inoculum origin on the biodegradation kinetics of hydrocarbons for five inocula from surface waters varying in urbanization and thus expected pre-exposure to petroleum hydrocarbons. A new biodegradation method for testing mixtures of hydrophobic chemicals at trace concentrations was demonstrated: Aqueous solutions containing 9 hydrocarbons were generated by passive dosing and diluted with surface water resulting in test systems containing native microorganisms exposed to test substances at ng-μg/L levels. Automated Headspace Solid Phase Microextraction coupled to GC-MS was applied directly to these test systems to determine substrate depletion relative to abiotic controls. Lag phases were generally less than 8 days. First order rate constants were within one order of magnitude for each hydrocarbon in four of the five waters but lower in water from a rural lake. The sequence of degradation between the 9 hydrocarbons showed similar patterns in the five waters indicating the potential for using selected hydrocarbons for benchmarking between biodegradation tests. Degradation half-times were shorter than or within one order of magnitude of BioHCwin predictions for 8 of 9 hydrocarbons. These results showed that location choice is important for biodegradation kinetics and can provide a relevant input to aquatic exposure and fate models. Graphical abstract: Highlights: Biodegradation of a mix of 9 hydrocarbons measured in five surface water samples. Passive dosing was used for preparation of low concentration test systems. Higher urbanization and/or bacterial density increased 1. order rate constants. Sequence of degradation of the hydrocarbons (HC) was consistent among five inocula. Half-times were less or within a factor of 10 from BioHCwin predictions for 8 HCs.