Repeatability of Oral Sugar Test Results, Glucagon‐Like Peptide‐1 Measurements, and Serum High‐Molecular‐Weight Adiponectin Concentrations in Horses. (24th May 2017)

• Record Type: Journal Article
• Authors: Frank, N.; Walsh, D.M.
• Is Part Of: Journal of veterinary internal medicine. Volume 31:Number 4(2017)
• Page Start: 1178
• Page End: 1187
• Publication Date: 2017-05-24
• Subjects: Endocrinology -- Equine metabolic syndrome -- Insulin -- Insulin dysregulation -- Laminitis; Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.14725
• Languages: English
• ISSNs: 0891-6640

Abstract

Abstract : Background: Repeatability of the oral sugar test (OST) has not been evaluated. Objectives: We hypothesized that OST glucose, insulin, active (aGLP‐1) and total (tGLP‐1) glucagon‐like peptide 1, and high‐molecular‐weight (HMW) adiponectin results would be repeatable. Animals: Fifty‐three horses from a Tennessee research facility (n = 23) and private practice in Missouri (n = 30), including animals with medical histories of equine metabolic syndrome. Methods: Two OSTs were performed 7–14 days apart and plasma glucose and insulin concentrations were measured at 0, 60, and 75 minutes; a positive result was defined as detection of an insulin concentration >45 μU/mL at 60 or 75 minutes. Plasma aGLP‐1 and serum tGLP‐1 concentrations at 75 minutes and serum HMW adiponectin concentrations at 0 minute were measured in the Missouri group. Bland‐Altman analyses were performed. Results: No adverse events were reported. Bland‐Altman analysis indicated mean ± SD bias of 1.5 ± 14.8 μU/mL (95% confidence interval [CI], −27.6 to 30.5 μU/mL) and 1.2 ± 16.1 μU/mL (95% CI, −30.4 to 32.8 μU/mL) for insulin concentrations at 60 and 75 minutes, respectively. There was 91 and 83% agreement in test interpretation between test days for OST insulin results for all horses in the Tennessee and Missouri groups, respectively. Conclusions and Clinical Importance: Repeatability of the OST was acceptable when values obtained from Bland‐Altman analyses were evaluated, and there was good agreement in binary (negative/positive) test interpretation for insulin concentrations. However, wide 95% CIs were detected for insulin concentrations.