Macrophage infiltration of electrospun polyester fibers. (22nd February 2017)

• Record Type: Journal Article
• Authors: Liu, Shen; Chen, Hua; Wu, Tianyi; Pan, Guoqing; Fan, Cunyi; Xu, Yaozeng; Cui, Wenguo
• Is Part Of: Biomaterials science. Volume 5:Number 8(2017:Aug.)
• Page Start: 1579
• Page End: 1587
• Publication Date: 2017-02-22
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c6bm00958a
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Ibuprofen (IBU)-loaded fibrous PLA membranes can prevent peritendinous adhesion/granuloma formation and inflammation by reducing macrophage infiltration. Abstract : Electrospun fibrous polylactide (PLA) membranes have been widely used for peritendinous anti-adhesion, but their degradation may lead to granuloma formation triggered by macrophages as the result of a foreign body reaction. To understand and solve this problem in peritendinous anti-adhesion, the effects of electrospun fibrous PLA membranes (PLA-M) and the mechanism by which macrophages elicit a foreign body reaction should be elaborated. Thus, the purpose of this study was to evaluate the ability of PLA-M and ibuprofen (IBU)-loaded fibrous PLA membranes (IBU/PLA-M) to prevent adhesion/granuloma formation around the tendon, and to understand the mechanism of macrophage infiltration. The results showed that PLA-M and IBU/PLA-M were porous and that IBU could be sustainably released from IBU/PLA-M. The adhesion and proliferation of RAW264.7 macrophages were worse on the surface of IBU/PLA-M than on PLA-M. After implantation around the tendon, IBU/PLA-M had higher anti-adhesion and tendon healing scores than PLA-M. Furthermore, PLA-M was able to promote macrophage infiltration, neovascularization, TNF-α expression and collagen III deposition to a greater extent than IBU/PLA-M. Consequently, these results show that macrophages can be triggered by PLA-M which subsequently leads to inflammation and granuloma formation, while IBU/PLA-M results in enhanced anti-inflammation and anti-adhesion effects when compared to PLA-M, by reducing macrophage infiltration.