Genes involved in leukotriene synthesis pathway are dynamically regulated during lung development in Rhesus monkeys. (July 2017)

• Record Type: Journal Article
• Authors: Xia, Wanmin; Xie, Liang; Cao, Bangrong; Cheng, Shujun; Wan, Huajing; Liu, Hanmin
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 122(2017)
• Page Start: 1
• Page End: 6
• Publication Date: 2017-07
• Subjects: RPKM reads per kilobase of transcript per million mapped reads -- FGF fibroblast growth factor -- ETS E26 transformation-specific or E-twenty-six -- LTs leukotrienes -- ALOX5 arachidonate 5-lipoxygenase -- LTA4 leukotriene A4 -- LTB4 leukotriene B4 -- LTC4 leukotriene C4 -- LTD4 leukotriene D4 -- LTE4 leukotriene E4 -- CysLTs cysteinyl leukotrienes -- PLA2 phospholipase A2 -- ALOX5AP arachidonate 5-lipoxygenase activating protein -- LTA4H leukotriene A4 hydrolase -- LTC4S leukotriene C4 synthase -- GGT γ-glutamyl transpeptidase -- DPEP dipeptidase; Leukotrienes -- Gene expression -- Lung development -- Rhesus monkey; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2017.06.008
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: Background: Leukotrienes play critical roles in many inflammatory lung diseases and several antagonists of their receptors have been used in the clinical settings. However, the physiological functions of leukotrienes in lung development are still unclear. Method: The expression levels of 34 genes involved in leukotriene synthesis and function pathway in the lungs of Rhesus monkey during different developmental time points were determined on a MiSeq platform and analyzed by the reads per kilobase of transcript per million mapped reads (RPKM) method. Results: The results showed that the expression levels of PLA2G1B, PLA2G10, PLA2G2D, ALOX5, and ALOX5AP increased dramatically in the lung of Rhesus monkey, reflecting the changes in the pulmonary environment after delivery. Additionally, the different expression patterns between molecules related to LTB4 and LTC4 synthesis suggested distinct roles of LTB4 and LTC4 in lung development. Finally, the constant expression of CysLT1 during the development process provided new information to the pharmaceutical basis of the use of leukotriene receptor antagonists in the clinical setting. Conclusion: The expression levels of several key genes involved in leukotriene synthesis changed dramatically during lung development in Rhesus monkeys, suggesting the potential roles of leukotrienes in lung development in this animal model. Highlights: The expressions of genes in LT synthesis pathway were evaluated in Rhesus monkeys. LTB4 could function in coping with the pulmonary environmental changes at birth. The different roles of LTB4 and CysLTs in lung development were suggested.