Glycoform of a newly identified pollen allergen, Cha o 3, from Chamaecyparis obtusa (Japanese cypress, Hinoki). (7th August 2017)

• Record Type: Journal Article
• Authors: Osada, Toshihiro; Maeda, Megumi; Tanabe, Chinatsu; Furuta, Kaori; Vavricka, Christopher J.; Sasaki, Eiji; Okano, Mitsuhiro; Kimura, Yoshinobu
• Is Part Of: Carbohydrate research. Volume 448(2017)
• Page Start: 18
• Page End: 23
• Publication Date: 2017-08-07
• Subjects: Antigenic N-glycan -- Pollen allergen -- Cha o 3 -- Japanese cypress -- Chamaecyparis obtuse; RP-HPLC reversed-phase HPLC -- SF-HPLC size-fractionation HPLC -- PA- pyridylamino -- Hex hexose -- HexNAc N-acetylhexosamine -- Pen pentose -- Deoxyhex deoxyhexose -- Man D-mannose -- GlcNAc N-acetyl-D-glucosamine -- Xyl D-xylose -- Fuc L-fucose -- Lea Lewis a (Galβ1-3(Fucα1-4)GlcNAc) -- PCT plant complex type -- M3FX Manα1-6(Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA -- GN1M3FX Manα1-6(GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA or GlcNAcβ1-2Manα1-6(Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA -- GN2M3FX GlcNAcβ1-2Manα1-6(GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA -- (Lea)1GN1M3FX GlcNAcβ1-2Manα1-6(Galβ1-3(Fucα1-4)GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA or Galβ1-3(Fucα1-4)GlcNAcβ1-2Manα1-6(GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA -- (Lea)2M3FX Galβ1-3(Fucα1-4)GlcNAcβ1-2Manα1-6(Galβ1-3(Fucα1-4)GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2017.05.005
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Cha o 3 is a newly found glycosylated allergen from Chamaecyparis obtusa (Japanese cypress) pollen. The deduced amino acid sequence of Cha o 3 indicates that this glycoallergen contains a cellulase domain and a number of putative N -glycosylation sites. However, the structures of N -glycans linked to Cha o 3 remain to be determined. In this study, therefore, we analyzed the glycoform of Cha o 3 and found that this glycoallergen carries exclusively plant complex-type N -glycans; major structures were GlcNAc2 Man3 Xyl1 Fuc1 GlcNAc2 (39%), Gal1 Fuc1 GlcNAc2 Man3 Xyl1 Fuc1 GlcNAc2 (14%), and Gal2 Fuc2 GlcNAc2 Man3 Xyl1 Fuc1 GlcNAc2 (25%). The glycoform of Cha o 3 bearing the Le a epitope is similar to those of Cry j1, Jun a 1, or Cup a 1, major glycoallergens in cedar or cypress pollens, and the predominant occurrence of GlcNAc2 Man3 Xyl1 Fuc1 GlcNAc2 is a common structural feature of glycoallergens from Cupressaceae pollens. Graphical abstract: Highlights: Glycoform of a newly identified cypress pollen allergen, Cha o3, was revealed. Glycoform of Cha o 3 is similar to those of other Cupressaceae pollen allergens. Lewis a epitope generally occurs on N -glycans of Cupressaceae pollen allergens.