A bioinformatics analysis of 3400 lytic polysaccharide oxidases from family AA9. (7th August 2017)

• Record Type: Journal Article
• Authors: Lenfant, Nicolas; Hainaut, Matthieu; Terrapon, Nicolas; Drula, Elodie; Lombard, Vincent; Henrissat, Bernard
• Is Part Of: Carbohydrate research. Volume 448(2017)
• Page Start: 166
• Page End: 174
• Publication Date: 2017-08-07
• Subjects: Lytic polysaccharide monooxygenases -- Evolution -- Bioinformatics -- Modular structure -- Regioselectivity -- Fungal lifestyle; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2017.04.012
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Lytic polysaccharide monooxygenases of family AA9 catalyze the oxidative cleavage of glycosidic bonds in cellulose and related polysaccharides. The N-terminal half of AA9 LPMOs displays a huge sequence variability that is in contradiction with the substrate simplicity so far observed for these enzymes. To understand the cause of the high multigenicity that prevails in the family, we have performed a clustering analysis of the N-terminal region of 3400 sequences of family AA9 LPMOs, and have evaluated the coincidence of the clusters with distal visible features that may accompany functional differences. A method based on local pairwise alignments was devised to avoid the pitfalls of a global multiple alignment. Our analysis allowed the definition of 64 clusters, which successfully segregated several visible features associated to LPMO family AA9, such as the presence of carbohydrate-binding modules, of modules of unknown function and of the conspicuous H → R substitution at the first residue of the histidine brace that holds the catalytic copper. Our analysis shows that the hypervariability of the N-terminal half of the AA9 sequences is not driven by random evolution as sequence similarity does not follow solely taxonomy. The results suggest that some clusters are perhaps able to target chitin instead of cellulose, and that preference for C1 or C4 oxidation (or lack thereof), does not appear to constitute a strong evolutionary constraint. On an evolutionary standpoint, there seems to be little constraints that apply to the N-terminal half of the sequences other than the conservation of the histidine brace. The weak evolutionary constraints that apply to the N-terminal half of AA9 LPMOs explain both their hypervariability and multigenicity. Graphical abstract: Highlights: A clustering of family AA9 LPMOs based on their variable N-terminal half is presented. Several distal sequence features of the AA9 family segregate in separate clusters. Regio-selectivity of the oxidation reaction does not coincide with clusters nor with phylogenetic groupings. Cluster profiles discriminate brown-rot from ectomycorrhizal fungi. The N-terminal half of AA9 sequences is under weak evolutionary constraints.