Structural characterization of wall and lipidated polysaccharides from Clostridium perfringens ATCC 13124. (7th August 2017)

• Record Type: Journal Article
• Authors: Vinogradov, Evgeny; Aubry, Annie; Logan, Susan M.
• Is Part Of: Carbohydrate research. Volume 448(2017)
• Page Start: 88
• Page End: 94
• Publication Date: 2017-08-07
• Subjects: Clostridium perfringens -- Polysaccharide -- Structure -- NMR spectroscopy; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2017.06.003
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Cell surface polysaccharides produced by C. perfringens ATCC 13124 were analyzed using NMR, chemical and immunological methods. Two distinct polymers were identified. The more abundant PS1 had a structure based on a polymer of β-mannosamine with a number of modifications, including varying levels of substitution at O-6 with PEtN, N-acetylation, and different linkages between monosaccharides. The shortest variant of PS1 represented a lipoteichoic acid. It contained only 1-4-linkages between ManNAc residues, minor branching α-Rib f, and glucosyl-glycerol at the reducing end, which was acylated with linear saturated fatty acids C16, C18, and C20 (dominant). Other non-lipidated variants of PS1 contained less P EtN, no α-Rib f, up to 50% 1-3-linkages, and up to 25% ManN with the free amino group. The minor polysaccharide PS2 had a linear regular structure with a -4-α-Rha-3-β-Gal-4-β-GalNAc3 P Cho- repeating unit, where P Cho indicates phosphocholine. Graphical abstract: Highlights: Clostridium perfringens strain 13124 produces polysaccharides of two types. The major polysaccharide is a homopolymer of mannosamine and can have a lipid part. The polysaccharides contain both positively and negatively charged groups in different ratios. No structure similarity was observed with C. perfringens polysaccharides described earlier.