Structure features and in vitro hypoglycemic activities of polysaccharides from different species of Maidong. (1st October 2017)

• Record Type: Journal Article
• Authors: Gong, Yajun; Zhang, Jie; Gao, Fei; Zhou, Jiewen; Xiang, Zhinan; Zhou, Chenggao; Wan, Luosheng; Chen, Jiachun
• Is Part Of: Carbohydrate polymers. Volume 173(2017)
• Page Start: 215
• Page End: 222
• Publication Date: 2017-10-01
• Subjects: Liriope spicata -- Ophiopogon japonicus -- Liriope muscari -- Polysaccharide -- Structure elucidation -- Anti-diabetic effect; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.05.076
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Polysaccharides LSP, LMP and OJP were isolated from three species of Maidong . The structure features of LSP, LMP and OJP were elucidated and compared. LSP, LMP and OJP possess high hypoglycemic activities in vitro . LSP, LMP and OJP could improve insulin resistant through PI3K/AKT pathway in IR HepG2 cells. LSP, LMP and OJP could be potential anti-diabetic polysaccharides. Abstract: Structures and in vitro hypoglycemic activities of polysaccharides from different species of Maidong were studied. The primary structures of polysaccharides were elucidated on the basis of GC, GC–MS, infrared, NMR and periodate oxidation-Smith degradation. Liriope spicata polysaccharide (LSP), Ophiopogon japonicus polysaccharide (OJP) and Liriope muscari polysaccharide (LMP) were composed of β -fructose and α-glucose. The average molecular weights of LSP, OJP and LMP were 4742, 4925 and 4138 Da with polydispersity indexes of 1.1, 1.2 and 1.1, respectively. The backbones of polysaccharides were formed by Fruf-(2→, →2)-Fruf-(6→, →6)-Glcp-(1→ and →1, 2)-Fruf-(6→ with a molar ratio of 5.0:18.2:1.0:5.3 (LSP), 6.8:15.8:1.0:5.8 (OJP), 8.3:12.3:1.0:3.9 (LMP), respectively. The RT-PCR and western blot analysis indicated that LSP, LMP and OJP increased the expression of PI3K, AKT, InsR, PPARγ and decreased the expression of PTP1B in mRNA level and protein level in IR HepG2 cells. Furthermore, glucose consumption was increased after treated with polysaccharides. These results revealed that LSP, OJP and LMP had potential anti-diabetic effects.