Effect of follicular dendritic cell secreted protein on gene expression of human periodontal ligament cells. (September 2017)

• Record Type: Journal Article
• Authors: Xiang, Lin; Xin, Na; Yuan, Ying; Hou, Xiaogang; Chen, Junwei; Wei, Na; Gong, Ping
• Is Part Of: Archives of oral biology. Volume 81(2017)
• Page Start: 151
• Page End: 159
• Publication Date: 2017-09
• Subjects: Follicular dendritic cell secreted protein (FDC-SP) -- Lentiviral vector -- Human periodontal ligament cells (hPDLCs) -- Microarray analysis -- Differential gene expression; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2017.05.005
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: FDC-SP's intrinsic functions in hPDLCs' phenotype expression was investigated upon microarray analysis. FDC-SP upregulates expressions of genes encoding cell-cycle proteins, blood-related and cell differentiation-related proteins in hPDLCs. FDC-SP downregulates expressions of genes encoding cytokine/growth factors, signal transduction and metabolism-related genes in hPDLCs. FDC-SP showed potentials to maintain PDL homeostasis, thus may contribute to periodontal would-healing processes. Abstract: Objective: The objective of this study was to investigate the specific roles of follicular dendritic cell secreted protein (FDC-SP), a protein exists in saliva, in the inhibition of calcium precipitation during periodontal regeneration, as well as affect phenotype expression of human periodontal ligament cells (hPDLCs) during the differentiation process. Design: To investigate this, we applied microarray technology to identify gene expression changes in hPDLCs transfected with FDC-SP and then clustered them according to their biological functions. Results: One hundred seventy-one genes were found differentially expressed by at least two-fold between FDC-SP -transfected and empty vector-transfected cells. Besides, genes encoding cell-cycle proteins, blood-related and cell differentiation-related proteins tended to be up-regulated after FDC-SP transfection, whereas cytokine/growth factors, signal transduction and metabolism-related genes tended to be down-regulated in hPDLCs overexpression FDC-SP. Conclusions: The present study investigated FDC-SP's roles in hPDLCs' phenotype expression, via comparing the gene expression profiles between FDC-SP -transfected hPDLCs and empty vector-transfected cells upon microarray analysis. hPDLCs overexpression FDC-SP appear to display different gene expression patterns. In all, these observations showed a potential of FDC-SP in the maintenance of PDL homeostasis and its ultimate contribution to periodontal would-healing processes.