Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3. Issue 6 (22nd April 2016)
- Record Type:
- Journal Article
- Title:
- Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3. Issue 6 (22nd April 2016)
- Main Title:
- Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3
- Authors:
- Li, Lei
Zhang, Si-Yu
Li, Yan-Mei
Chen, Yong-Xiang - Abstract:
- Abstract : SUMO-fused and intein-fused protein expression systems have been combined to prepare a dual-color labeled ubiquitin sensor for detecting UCH-L3's activity. Abstract : A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.
- Is Part Of:
- Molecular bioSystems. Volume 12:Issue 6(2016:Jun.)
- Journal:
- Molecular bioSystems
- Issue:
- Volume 12:Issue 6(2016:Jun.)
- Issue Display:
- Volume 12, Issue 6 (2016)
- Year:
- 2016
- Volume:
- 12
- Issue:
- 6
- Issue Sort Value:
- 2016-0012-0006-0000
- Page Start:
- 1764
- Page End:
- 1767
- Publication Date:
- 2016-04-22
- Subjects:
- Molecular biology -- Periodicals
Biochemistry -- Periodicals
571.7405 - Journal URLs:
- http://www.rsc.org/Publishing/Journals/mb/index.asp ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c6mb00165c ↗
- Languages:
- English
- ISSNs:
- 1742-206X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 5900.798350
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 2914.xml