Complete Genome Sequence of a Novel Wild tomato mosaic virus Isolate Infecting Nicotiana tabacum in China. Issue 9 (22nd September 2015)

• Record Type: Journal Article
• Authors: Sun, Hangjun; Liu, Wei; Yang, Jinguang; Wang, Fenglong; Shen, Lili; Li, Ying; Sun, Dongmei; Qian, Yumei
• Is Part Of: Journal of phytopathology. Volume 164:Issue 9(2016:Sep.)
• Page Start: 686
• Page End: 690
• Publication Date: 2015-09-22
• Subjects: Nicotiana tabacum -- Potyvirus -- sequence analysis -- Wild tomato mosaic virus; Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12443
• Languages: English
• ISSNs: 0931-1785

Abstract

Abstract: Virus particles of approximately 740–760 nm in length and 13 nm in diameter were observed from a diseased Nicotiana tabacum (tobacco) plant in Sichuan Province, China. The complete genomic sequence of the virus isolate XC1 was determined to contain 9659 nucleotides without 3′ terminal poly(A) tail. XC1 has a genome typical of members of the genus Potyvirus, encoding a large polyprotein of 3075 amino acids. Putative proteolytic cleavage sites and a number of well characterized functional motifs were identified by sequence comparisons with those of known potyviruses. Sequence comparison revealed that XC1 shared the highest level of nucleotide sequence identity (76.5%) with Wild tomato mosaic virus (WTMV). Phylogenetic analysis showed that XC1 was closely related to the WTMV Guangdong isolate with an identity of 94.3% between CP gene sequence of the two viruses. We thus named XC1 WTMV‐XC‐1 as a novel isolate of WTMV. The full sequence of WTMV‐XC‐1 may serve as a basis for future investigations on the gene diversity of WTMV.