Neonatal hypoxia‐ischemia in rat increases doublecortin concentration in the cerebrospinal fluid. (4th July 2017)

• Record Type: Journal Article
• Authors: Brégère, Catherine; Fisch, Urs; Sailer, Martin H.; Lieb, Wolfgang S.; Chicha, Laurie; Goepfert, Fabienne; Kremer, Thomas; Guzman, Raphael
• Is Part Of: European journal of neuroscience. Volume 46:Number 2(2017)
• Page Start: 1758
• Page End: 1767
• Publication Date: 2017-07-04
• Subjects: biological marker -- neurodevelopmental brain injury -- neurogenesis -- rodent -- stroke severity; Nervous system -- Periodicals; 612.8
• DOI: 10.1111/ejn.13612
• Languages: English
• ISSNs: 0953-816X

Abstract

Abstract: Doublecortin (DCX) is a microtubule‐associated protein widely used as an indicator of neurogenesis in immunohistochemical analyses of the postmortem adult brain. A recent study reported that DCX can be quantified in the cerebrospinal fluid (CSF) from healthy rats between postnatal day 0 (P0) and P30. However, it is currently unclear whether the concentration of DCX in the CSF (CSF‐DCX) may represent a measure of endogenous neurogenesis. To address this question, this study examined the impact of a neonatal hypoxic‐ischemic (HI) brain injury, known to induce neurogenesis, on CSF‐DCX. HI was elicited at P7 in Sprague–Dawley rat neonates, and CSF was collected serially from the cisterna magna at P5 and P10, or at P10 and P15. A sandwich immunoassay was used to measure CSF‐DCX. Brains from P10 neonates were analyzed immunohistochemically for neurogenesis and cell death markers. Mean CSF‐DCX was significantly higher in HI‐ than in sham‐exposed animals, at both P10 and P15. In the HI group at P10, CSF‐DCX and stroke severity correlated positively. DCX immunoreactivity was increased in the ipsilateral neurogenic niches from the P10 HI brains in comparison with that of shams. The number of proliferative DCX‐positive cells was higher in the ipsilateral hippocampal subgranular zone (SGZ) than in the HI contralateral or sham SGZ. Thus, neonatal HI brain injury disrupts the developmental time‐course of DCX levels in the CSF. Our data suggest that the increased concentration of DCX in the CSF after neonatal HI is the result of both cellular injury and increased neurogenesis. Abstract : The main goal of this report was to examine whether the concentration of the protein doublecortin (DCX) in the CSF (CSF‐DCX) reflects endogenous neurogenesis in the brain. Using a rat model of neonatal hypoxia‐ischemia (HI), we found that CSF‐DCX was a measure of both stroke severity and HI‐induced neurogenesis. ** = P < 0.01, **** = P < 0.0001.