Influence of nitrogen depletion in the growth of N. oleoabundans on the release of cellular components after beadmilling. (August 2016) - Günerken, Emre
Highlights: Pellet and supernatant were analyzed to evaluate algae cell disruption. Energy consumption per kg of released product was calculated. Nitrogen depletion results in a higher release ratio of biochemical components. Nitrogen depletion results in a lower energy consumption per kg of released product. Abstract: Cell disruption by bead milling of Neochloris oleoabundans grown under nitrogen repleted (NR) and nitrogen depleted (ND) conditions was evaluated based on the release of biochemicals and biomass to the supernatant. Additionally, energy consumption for cell disruption was calculated per kg of released component. Bead milling of NR cells resulted on average in 34% (w/w) release of biochemicals into the supernatant, with a similar composition as the untreated microalgal biomass. With ND cells, the release was higher and more selective, i.e. 57%, 59%, 68% and 56% (w/w) of respectively biomass, proteins, carbohydrates and lipids whereof 92%, 57% and 46% (w/w) respectively of phospho-, glyco- and neutral lipids.