Development of a rechargeable optical hydrogen peroxide sensor – sensor design and biological application. Issue 14 (17th May 2016)

• Record Type: Journal Article
• Authors: Koren, Klaus; Jensen, Peter Ø.; Kühl, Michael
• Is Part Of: Analyst. Volume 141:Issue 14(2016)
• Page Start: 4332
• Page End: 4339
• Publication Date: 2016-05-17
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c6an00864j
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Measure and recharge! A reversible sensor concept enables repetitive and quantitative measurement of H2 O2 with high spatial and temporal resolution in environmental and biomedical samples. Abstract : Hydrogen peroxide (H2 O2 ) is an important member of the reactive oxygen species (ROS) family. Among ROS, H2 O2 is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H2 O2 within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H2 O2 concentrations ranging from 1–100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H2 O2 can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H2 O2 concentration profile measurements in natural photosynthetic biofilms under light stress reaching H2 O2 concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H2 O2 during stimulation of neutrophils.