Bioactive SiO2@Ru nanoparticles for osteogenic differentiation of mesenchymal stem cells via activation of Akt signaling pathways. Issue 25 (25th January 2016)

• Record Type: Journal Article
• Authors: Liu, Ying; Huang, Na; Yu, Yunfei; Zheng, Chuping; Deng, Ning; Liu, Jie
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 25(2016)
• Page Start: 4389
• Page End: 4401
• Publication Date: 2016-01-25
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c5tb01898f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : The surface chemistry of materials has an interactive influence on cell behavior. Abstract : The surface chemistry of materials has an interactive influence on cell behavior. It is now well established that surface chemistry can affect cell adhesion, proliferation, and differentiation. Although amino (NH2 )-terminated surfaces generated by the modification of nanoparticles with silane can promote osteogenic differentiation of mesenchymal stem cells (MSCs), how silica surfaces with ruthenium nanoparticles (SiO2 @Ru) act on MSCs remains largely unknown. A concentration of 5 μg mL −1 aminopropyltriethoxysilane (APTS)-modified SiO2 nanoparticles (SiO2 –NH2 ) or SiO2 @Ru was nontoxic to MSCs, based on MTT and apoptosis assays. In addition, SiO2 –NH2 and SiO2 @Ru did not affect the surface phenotype or morphology of MSCs. SiO2 @Ru can be used to trigger the differentiation of MSCs into osteocytes, minimising the need for exogenous biological supplementation. TEM images revealed that SiO2 @Ru might interact with proteins located in the cytoplasm, which would have a further impact on subsequent cellular signaling pathways. Activation of Akt signaling pathways was observed in MSCs cultured with SiO2 @Ru and these enhancement effects could be blocked by the Akt inhibitor LY294002. SiO2 @Ru exhibited in vitro osteocompatibility that surpassed that of SiO2 –NH2, as well as supporting the proliferation and differentiation of MSCs. This demonstrates the potential of SiO2 @Ru for use in bone regeneration.