A pneumatic pressure-driven multi-throughput microfluidic circulation culture system. Issue 12 (27th May 2016)

• Record Type: Journal Article
• Authors: Satoh, T.; Narazaki, G.; Sugita, R.; Kobayashi, H.; Sugiura, S.; Kanamori, T.
• Is Part Of: Lab on a chip. Volume 16:Issue 12(2016)
• Page Start: 2339
• Page End: 2348
• Publication Date: 2016-05-27
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c6lc00361c
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We present a pneumatic pressure-driven multi-throughput medium circulation culture system with pipette-friendly liquid handling and a detachable interface for microscope observation. Abstract : Here, we report a pneumatic pressure-driven microfluidic device capable of multi-throughput medium circulation culture. The circulation culture system has the following advantages for application in drug discovery: (i) simultaneous operation of multiple circulation units, (ii) use of a small amount of circulating medium (3.5 mL), (iii) pipette-friendly liquid handling, and (iv) a detachable interface with pneumatic pressure lines via sterile air-vent filters. The microfluidic device contains three independent circulation culture units, in which human umbilical vein endothelial cells (HUVECs) were cultured under physiological shear stress induced by circulation of the medium. Circulation of the medium in the three culture units was generated by programmed sequentially applied pressure from two pressure-control lines. HUVECs cultured in the microfluidic device were aligned under a one-way circulating flow with a shear stress of 10 dyn cm −2 ; they exhibited a randomly ordered alignment under no shear stress and under reciprocating flow with a shear stress of 10 dyn cm −2 . We also observed 2.8- to 4.9-fold increases in expression of the mRNAs of endothelial nitric oxide synthase and thrombomodulin under one-way circulating flow with a shear stress of 10 dyn cm −2 compared with conditions of no shear stress or reciprocating flow.