Regional quantification of myocardial mechanics in rat using 3D cine DENSE cardiovascular magnetic resonance. (8th May 2017)

• Record Type: Journal Article
• Authors: Zhang, Xiaoyan; Liu, Zhan‐Qiu; Singh, Dara; Wehner, Gregory J.; Powell, David K.; Campbell, Kenneth S.; Fornwalt, Brandon K.; Wenk, Jonathan F.
• Is Part Of: NMR in biomedicine. Volume 30:Number 8(2017:Aug.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-05-08
• Subjects: 3D cine DENSE CMR -- rat -- strain -- torsion; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.3733
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : Rat models have assumed an increasingly important role in cardiac research. However, a detailed profile of regional cardiac mechanics, such as strains and torsion, is lacking for rats. We hypothesized that healthy rat left ventricles (LVs) exhibit regional differences in cardiac mechanics, which are part of normal function. In this study, images of the LV were obtained with 3D cine displacement encoding with stimulated echoes (DENSE) cardiovascular magnetic resonance in 10 healthy rats. To evaluate regional cardiac mechanics, the LV was divided into basal, mid‐ventricular, and apical regions. The myocardium at the mid‐LV was further partitioned into four wall segments (i.e. septal, inferior, lateral, and anterior) and three transmural layers (i.e. sub‐endocardium, mid‐myocardium, and sub‐epicardium). The six Lagrangian strain components (i.e. E rr, E cc, E ll, E cl, E rl, and E cr ) were computed from the 3D displacement field and averaged within each region of interest. Torsion was quantified using the circumferential‐longitudinal shear angle. While peak systolic E cl differed between the mid‐ventricle and apex, the other five components of peak systolic strain were similar across the base, mid‐ventricle, and apex. In the mid‐LV myocardium, E cc decreased gradually from the sub‐endocardial to the sub‐epicardial layer. E ll demonstrated significant differences between the four wall segments, with the largest magnitude in the inferior segment. E rr was uniform among the four wall segments. E cl varied along the transmural direction and among wall segments, whereas E rl differed only among the wall segments. E rc was not associated with significant variations. Torsion also varied along the transmural direction and among wall segments. These results provide fundamental insights into the regional contractile function of healthy rat hearts, and form the foundation for future studies on regional changes induced by disease or treatments. Abstract : Based on 3D cine DENSE measurements, peak systolic E cc and E ll varied among the three transmural layers (i.e. sub‐endocardium, mid‐myocardium, and sub‐epicardium) and among the four wall segments (i.e. septal, inferior, lateral, and anterior), respectively, at the mid‐ventricle of healthy rats. Peak systolic E cl and related ventricular torsion exhibited notable differences along the transmural direction and among wall segments.