Inside Back Cover: Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity (ChemBioChem 14/2017). (28th June 2017)

• Record Type: Journal Article
• Authors: Babkova, Petra; Sebestova, Eva; Brezovsky, Jan; Chaloupkova, Radka; Damborsky, Jiri
• Is Part Of: Chembiochem. Volume 18:Number 14(2017)
• Page Start: 1458
• Page End: 1458
• Publication Date: 2017-06-28
• Subjects: ancestral sequence reconstruction -- haloalkane dehalogenase -- protein engineering -- robustness -- substrate specificity; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201700335
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : The inside back cover picture shows the reconstruction of the ancestral sequence of haloalkane dehalogenases. Ancestral enzymes exhibited significantly enhanced thermodynamic stability compared to extant ones, as well as higher specific activities with a preference for short, multisubstituted halogenated substrates. Ancestral sequence reconstruction represents a powerful strategy for constructing highly stable and active catalysts that can further serve as robust templates for directed evolution. More information can be found in the full paper by R. Chaloupkova, J. Damborsky, et al. onpage 1448 in Issue 14, 2017 (DOI:10.1002/cbic.201700197 ).