A Carborane‐Containing Fluorophore as a Stain of Cellular Lipid Droplets. Issue 14 (22nd June 2017)

• Record Type: Journal Article
• Authors: Wu, Andrew; Kolanowski, Jacek L.; Boumelhem, Badwi B.; Yang, Kylie; Lee, Rebecca; Kaur, Amandeep; Fraser, Stuart T.; New, Elizabeth J.; Rendina, Louis M.
• Is Part Of: Chemistry, an Asian journal. Volume 12:Issue 14(2017)
• Page Start: 1704
• Page End: 1708
• Publication Date: 2017-06-22
• Subjects: carboranes -- cellular markers -- coumarins -- fluorophores -- lipid droplets; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201700423
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane‐containing coumarin, 5, which is sufficiently lipophilic to localise in cellular lipid droplets. In non‐polar solvents which show comparable polarities to those of a lipid environment, compound 5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging. Compound 5 can stain lipid droplets in ex vivo adipocytes as well as in cultured cells, and can be utilised in flow cytometry as well as confocal microscopy. Abstract : High selectivity for lipids : The solvent‐dependent photophysical properties and lipophilic nature of a novel carborane‐coumarin fluorophore results in exquisite selectivity toward cellular lipid droplets.