Electrochemical Immunosensor for Sensitive Determination of TGF β1 in Urine. (2017)

• Record Type: Journal Article
• Authors: Sánchez-Tirado, E.; González-Cortés, A.; Yánez-Sedeño, P.; Pingarrón, J.M.
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 81
• Page End: 84
• Publication Date: 2017
• Subjects: Transforming Growth Factor–β1 -- TGF–β1 -- electrochemical immunosensor -- signal amplification -- urine; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.035
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: The first amperometric immunosensor for the quantification of TGF– β 1 is described in this work. The immunosensor design involves disposable devices using carboxylic acid–functionalized magnetic microparticles supported onto screen–printed carbon electrodes and covalent immobilization of the specific antibody for TGF– β 1 using Mix&Go TM polymer. A sandwich–type immunoassay was performed using peroxidase–labeled streptavidin (Poly–HRP–Strept) polymer for signal amplification. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF– β 1 (LOD 10 pg/mL). The usefulness of the immunosensor was evaluated by analyzing spiked urine at different pg/mL concentration levels.