Electrocatalytic Evaluation of a Horseradish Peroxidase Biosensor Based on a Novel Bi-Ag Bimetallic Nanocomposite. (2017)

• Record Type: Journal Article
• Authors: van der Horst, Charlton; Silwana, Bongiwe; Iwuoha, Emmanuel; Somerset, Vernon
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 179
• Page End: 182
• Publication Date: 2017
• Subjects: Differential pulse voltammetry -- Horseradish peroxidase -- Hydrogen peroxide -- Bismuth-silver nanoparticles -- Cyclic voltammetry; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.077
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: The present work illustrates the construction and application of bismuth-silver bimetallic nanoparticles (Bi-AgNPs) in biosensor construction for the detection of peroxide (H2 O2 ). The biosensor was constructed by drop-casting of horseradish peroxidase onto the Bi-AgNPs/GCE sensor. Impedance analysis revealed that the R ct value for the bare GCE obtained was 2.548 × 10 3 Ω and increased to 5.768 × 10 3 Ω for the HRP/Bi-AgNPs/GCE biosensor. This increased value of R ct indicated hindrance to the electron transfer, providing evidence for the immobilization of insulating HRP on the Bi-AgNPs/GCE surface. The HRP/Bi-AgNPs/GCE biosensor was successfully applied for sensitive detection of H2 O2 by differential pulse voltammetry. Well defined peaks were observed with a low linearity range from 0.02 × 10 −6 to 1.0 × 10 −6 M ( R 2 = 0.991), with a limit of detection of 0.06 μM. The results obtained for the HRP/Bi-AgNPs/GCE biosensor compared well within the range of similar biosensors reported.