Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation. (29th May 2017)

• Record Type: Journal Article
• Authors: Noguchi, Kanami; Ishikawa, Ryota; Kawaguchi, Masahumi; Miyoshi, Kanako; Kawasaki, Takahiko; Hirata, Tatsumi; Fukui, Makiko; Kuratani, Shigeru; Tanaka, Mikiko; Murakami, Yasunori
• Other Names: KAWAKAMI K. guestEditor.; MURAKAMI Y. guestEditor.
• Is Part Of: Development growth and differentiation. Volume 59:Number 4(2017)
• Page Start: 270
• Page End: 285
• Publication Date: 2017-05-29
• Subjects: amniote -- axon guidance molecule -- limb bud -- spinal nerve; Embryology -- Periodicals; Developmental biology -- Periodicals; Growth -- Periodicals; 574.3
• DOI: 10.1111/dgd.12364
• Languages: English
• ISSNs: 0012-1592

Abstract

Abstract : Paired limbs were acquired in the ancestor of tetrapods and their morphology has been highly diversified in amniotes in relation to the adaptive radiation to the terrestrial environment. These morphological changes may have been induced by modification of the developmental program of the skeletal or muscular system. To complete limb modification, it is also important to change the neuronal framework, because the functions of the limbs rely on neural circuits that involve coordinated movement. Previous studies have shown that class 3 semaphorins (Sema3 semaphorins), which act as repulsive axonal guidance cues, play a crucial role in the formation of the peripheral nerves in mice. Here, we studied the expression pattern of Sema3A orthologues in embryos of developing amniotes, including mouse, chick, soft‐shelled turtle, and ocelot gecko. Sema3A transcripts were expressed in restricted mesenchymal parts of the developing limb primordium in all animals studied, and developing spinal nerves appeared to extend through Sema3A ‐negative regions. These results suggest that a Sema3A ‐dependent guidance system plays a key role in neuronal circuit formation in amniote limbs. We also found that Sema3A partially overlapped with the distribution of cartilage precursor cells. Based on these results, we propose a model in which axon guidance and skeletogenesis are linked by Sema3A ; such mechanisms may underlie functional neuron rearrangement during limb diversification. Abstract : A Sema3A ‐dependent guidance system plays a key role in the development of amniote limb innervations. We propose a model that axon guidance of spinal nerves in amiotes is linked to skeltogenesis via repulsive guidance of Sema3A .