Bio-inspired peptide decorated dendrimers for a robust antibacterial coating on hydroxyapatite. Issue 29 (3rd July 2017)

• Record Type: Journal Article
• Authors: Gou, Yaping; Yang, Xiao; He, Libang; Xu, Xinyuan; Liu, Yanpeng; Liu, Yuebo; Gao, Yuan; Huang, Qin; Liang, Kunneng; Ding, Chunmei; Li, Jiyao; Zhao, Changsheng; Li, Jianshu
• Is Part Of: Polymer chemistry. Volume 8:Issue 29(2017)
• Page Start: 4264
• Page End: 4279
• Publication Date: 2017-07-03
• Subjects: Polymers -- Periodicals; Macromolecules -- Periodicals; Polymerization -- Periodicals; 547.705
• DOI: 10.1039/c7py00811b
• Languages: English
• ISSNs: 1759-9954

Abstract

Abstract : SSP-PAMAM-NH2 inspired by the salivary statherin protein can tightly adsorb on the HA surface to achieve long-term antibacterial activity. Abstract : Bacterial colonization on implanted biomaterials remains a clinically significant problem. In order to achieve relatively long-term antibacterial activity and reduce the incidence of infections associated with the use of biomaterials, a salivary statherin protein (SSP) inspired poly(amidoamine) dendrimer (SSP-PAMAM-NH2 ) was synthesized and characterized. PAMAM-NH2 has numerous peripheral amino groups, and thus possesses effective antibacterial activity. The SSP bio-inspired peptide sequence DDDEEKC was conjugated to PAMAM-NH2 since it has a strong capability of adsorbing on hydroxyapatite (HA). Moreover, SSP-PAMAM-NH2 is a zwitterionic polymer possessing cationic amino groups and anionic carboxylic groups, thus it can form aggregates by intermolecular electrostatic interactions, thereby promoting its adsorption on HA. Adsorption tests by ATR-IR, UV, QCM-D, and CLSM, all indicated that SSP-PAMAM-NH2 can tightly adsorb on the HA surface. We found that even after being incubated in PBS for 4 weeks, the SSP-PAMAM-NH2 treated HA disks still retained stable antibacterial activity, while the inhibitory impact of PAMAM-NH2 treated disks had disappeared. Animal experiments also demonstrated that SSP-PAMAM-NH2 could significantly reduce infection of HA implanted into the medullary cavity of rats.