Culture Mediums and Buffer Effect on Screen-printed Carbon Electrodes for Continuous Voltammetric Monitoring of in vitro Cell Cultures Lactate Production. (2017)

• Record Type: Journal Article
• Authors: Rosati, G.; Scaramuzza, M.; Rotilio, V.; Monaco, L.; Pasqualotto, E.; Campolo, F.; De Toni, A.; Reggiani, C.; Naro, F.; Paccagnella, A.
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 246
• Page End: 247
• Publication Date: 2017
• Subjects: lactic acid -- cell medium -- EIS -- cyclic voltammetry -- DMEM -- NADH; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.105
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: In this work we characterize and explain the difficulties related to voltammetric measurements in DMEM (Dulbecco's Modified Eagle's Medium) on widespread, commercially available Dropsens carbon screen-printed electrodes. The DMEM effects on the electrodes' surface is investigated by EIS, before and after 75 CV cycles. HBRS (Hepes Buffered Ringer's Solution) is proposed as alternative cell medium, and validated for the voltammetric monitoring of cell culture lactate production.