Cell type-selective imaging and profiling of newly synthesized proteomes by using puromycin analogues. Issue 60 (12th July 2017)

• Record Type: Journal Article
• Authors: Du, Shubo; Wang, Danyang; Lee, Jun-Seok; Peng, Bo; Ge, Jingyan; Yao, Shao Q.
• Is Part Of: Chemical communications. Volume 53:Issue 60(2017)
• Page Start: 8443
• Page End: 8446
• Publication Date: 2017-07-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc04536k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. Abstract : We have developed a versatile antibody-assisted strategy for the imaging and profiling of newly synthesized proteomes in a cell-specific manner. This strategy remained highly selective even in heterogeneous co-cultured cells, thus enabling labeling and enrichment of nascent proteomes from targeted cells without the need for physical separation.