A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering. (2017)

• Record Type: Journal Article
• Authors: Özkayar, Gürhan; Yalçın, Yağmur Demircan; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 177
• Page End: 178
• Publication Date: 2017
• Subjects: Dielectrophoresis (DEP) -- rare cell enrichment -- blood -- and cell slide; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.076
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.