Simplified Immuno-assay for Rapid Label-free Dengue Serotype Diagnosis: Suppression of Non-specific Binding Interference. (2017)

• Record Type: Journal Article
• Authors: Loureiro, F.C.L.; Lima, A.M.N.; Roque, R.; de Figueiredo, R.M.P.; Melcher, E.U.K.; Borre, M.; Thirstrup, C.; Neff, H.
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 67
• Page End: 69
• Publication Date: 2017
• Subjects: immuno-assay -- dengue virus detection -- non-specific binding; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.031
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: An easy to implement immunoassay for rapid dengue virus (DENV) serotype diagnosis in the early infection stage is reported. The four-layer assay is immobilized onto a thin gold film and relies on a polymer biochip for optical surface plasmon resonance sensing and detection. The protocol comprises Neutravidin-Biotin mediated DENV monoclonal antibody (MABD ) attachment as the functionalized sensing element. The MABD -DENV complex formation results in a pronounced thickness change that is optically detected in real time. The protocol is applicable in presence of strong non-specific binding interference. False positive indications for negative serum and blood control samples were not observed. The method can be modified towards detection of other viruses infections.