SERS-active Metal-dielectric Nanostructures Integrated in Microfluidic Devices for Ultra-sensitive Label-free miRNA Detection. (2017)

• Record Type: Journal Article
• Authors: Chiadò, A.; Novara, C.; Lamberti, A.; Geobaldo, F.; Giorgis, F.; Rivolo, P.
• Is Part Of: Procedia technology. Volume 27(2016)
• Page Start: 37
• Page End: 38
• Publication Date: 2017
• Subjects: Surface Enhanced Raman Scattering -- miRNA -- ELISA -- optofluidic biosensor -- metal-dielectric nanostructures -- porous silicon; Technology -- Congresses; Technology -- Periodicals; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Technology; Conference proceedings; Periodicals; 605
• DOI: 10.1016/j.protcy.2017.04.018
• Languages: English
• ISSNs: 2212-0173

Abstract

Abstract: In this work, silver decorated porous silicon membranes integrated in a polydimethylsiloxane multi-chamber microfluidic chip were functionalized with DNA-probes and used for the detection of miRNA by Surface-enhanced Raman Scattering analysis. An innovative biological protocol has been designed: the probe was divided in two short pieces that interact before and after the miRNA incubation. The optofluidic biosensor was applied for the label-free detection of miRNA sequences at in vivo concentrations.