A novel two-photon fluorescent probe for hydrogen sulfide in living cells using an acedan–NBD amine dyad based on FRET process with high selectivity and sensitivity. (21st June 2017) - Tang, Yao
Abstract : The first NBD amine based two-photon fluorescence probeL using a FRET strategy was developed for the H2 S detecting in vitro and in vivo . The probeL not only afforded high selectivity and sensitivity for H2 S detecting, but also dispalyed a linear response to H2 S with a low detection limit 24 nM. Abstract : Two-photon imaging, due to its unique advantage to utilize near-infrared light as the excitation source, has emerged as an important tool for biomedical research. Herein, the first NBD amine based two-photon fluorescence probeL using a FRET strategy was developed for detecting H2 S in vitro and in vivo . The probeL not only afforded high selectivity and sensitivity for the detection of H2 S, but also displayed a linear response to H2 S with a low detection limit of 24 nM. Furthermore, the living cell experiments indicated thatL can be effective for the both exogenous and endogenous detection of H2 S inside living cells by two-photon fluorescence imaging.