Determination of lacquer contained in samples of cultural relics by enzyme-linked immunosorbent assay. (14th June 2017)

• Record Type: Journal Article
• Authors: Wu, Meng; Zhang, Bingjian; Sun, Guoping; Jiang, Leping
• Is Part Of: New journal of chemistry. Volume 41:Number 14(2017)
• Page Start: 6226
• Page End: 6231
• Publication Date: 2017-06-14
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c7nj00831g
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : As an environmentally friendly natural polymer material, lacquer is durable, anti-bacterial, corrosion-resistant and decorative. Abstract : As an environmentally friendly natural polymer material, lacquer is durable, anti-bacterial, corrosion-resistant and decorative. In China, the use of lacquer has a long history. Traditional methods of detecting lacquer target on urushiol, however, are highly influenced by impurities and cannot detect traces of lacquer in samples of cultural relics. In this study, we attempted to detect lacquer using enzyme-linked immunosorbent assay (ELISA) after extracting and purifying glycoprotein from lacquer sap and obtained a specific anti-lacquer polyclonal antibody. Our experimental results showed that ELISA was highly effective, specific, and could detect traces of lacquer without being affected by impurities. This method was also applied to the detection of lacquerware cultural relics.