Crotonaldehyde induces autophagy-mediated cytotoxicity in human bronchial epithelial cells via PI3K, AMPK and MAPK pathways. (September 2017)

• Record Type: Journal Article
• Authors: Wang, Limeng; Li, Xiang; Yang, Zhihua; Pan, Xiujie; Liu, Xingyu; Zhu, Maoxiang; Xie, Jianping
• Is Part Of: Environmental pollution. Volume 228(2017)
• Page Start: 287
• Page End: 296
• Publication Date: 2017-09
• Subjects: Crotonaldehyde -- Autophagy -- PI3K -- AMPK -- MAPK; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2017.03.083
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Crotonaldehyde is an ubiquitous hazardous pollutant in the environment which can be produced naturally, artificially and endogenously. Acute exposure of crotonaldehyde was reported to induce severe lung injury in humans and experimental animals. However, the exact toxicity mechanisms of crotonaldehyde in organisms have not been fully explored. In the present study, we explored the role autophagy played in the cytotoxicity induced by crotonaldehyde in human bronchial epithelial cells (BEAS-2B), and the pathways that mediated autophagy, including the phosphatidylinositol 3-kinase (PI3K) pathway, the AMP-activated protein kinase (AMPK) pathway and the mitogen-activated protein kinase (MAPK) pathways, were examined and validated. We found that crotonaldehyde induced cytotoxicity and autophagy simultaneously in BEAS-2B cells, and blockage of autophagic flux significantly elevated the viability of BEAS-2B exposed to high concentrations of crotonaldehyde. Crotonaldehyde down-regulated the activity of PI3K pathway, and elevated the activities of AMPK and MAPK pathways. Pretreatment of specific agonist or antagonist of these pathways could inhibit autophagy and partly improve the viability. These results suggested that acute exposure of crotonaldehyde induced cell death mediated by autophagy, which might be helpful to elucidate the toxicity mechanisms of crotonaldehyde and contribute to environmental and human health risk assessment. Graphical abstract: Highlights: Crotonaldehyde induced acute cytotoxicity in human bronchial epithelial cells. Crotonaldehyde elevated autophagy in human bronchial epithelial cells. Blockage of autophagic flux attenuated crotonaldehyde induced cytotoxicity. Autophagy was induced via PI3K, AMPK and MAPK pathways. Block of PI3K, AMPK and MAPK pathways partly promoted cellular survival. Abstract : Crotonaldehyde induces autophagy-mediated cytotoxicity in BEAS-2B cells via PI3K, AMPK and MAPK pathways.