Uterus tolerance to extended cold ischemic storage after auto-transplantation in ewes. (July 2017)

• Record Type: Journal Article
• Authors: Tricard, Jérémy; Ponsonnard, Sébastien; Tholance, Yannick; Mesturoux, Laura; Lachatre, Denis; Couquet, Claude; Terro, Faraj; Yardin, Catherine; Marquet, Pierre; Piccardo, Alessandro; Gauthier, Tristan
• Is Part Of: European journal of obstetrics, gynecology, and reproductive biology. Volume 214(2017)
• Page Start: 162
• Page End: 167
• Publication Date: 2017-07
• Subjects: Cold ischemia time -- Ewe -- Ischemia reperfusion -- Uterus transplantation -- Fertility; Obstetrics -- Periodicals; Gynecology -- Periodicals; Reproductive health -- Periodicals; Gynecology -- Periodicals; Obstetrics -- Periodicals; Reproduction -- Periodicals; Obstétrique -- Périodiques; Gynécologie -- Périodiques; Reproduction -- Périodiques; Verloskunde; Gynaecologie; Voortplanting (biologie); Gynecology; Obstetrics; Reproduction; Electronic journals; Periodicals; Electronic journals; 618.05
• DOI: 10.1016/j.ejogrb.2017.05.013
• Languages: English
• ISSNs: 0301-2115

Abstract

Abstract: Objective: To assess how the uterus tolerates extended cold ischemic storage before auto-transplantation in ewes. Study design: Fourteen uterine auto-transplantations were performed in ewes from November 2014 to June 2015 at the Analysis and Research Laboratory of Limoges, France. The animals were divided into 2 groups: 7 after 3 h of cold ischemia timeand 7 after 24 h. Transplant was assessed ≥8 days after transplantation. Histology and apoptosis analyses (TUNEL method and indirect immunohistochemistry of cleaved Caspase 3) were performed before uterus retrieval (control), after 90 min following reperfusion and ≥8 days after transplantation. Results: Twelve uterine auto-transplantations were successfully performed. The histological analysis at 90 min following reperfusion revealed a moderate inflammation of the endometrium and serosa in the 3-h group and severe inflammation in the 24-h group, but no significant apoptotic signal was found in either group. Seven ewes were alive at ≥8 days after transplantation: the macroscopic and histological analyses revealed two viable uteri in the 3-h group and three in the 24-h group. In each group one uterus was necrotic. Conclusion: These first results in ewes suggest that the uterus is an organ with a good tolerance to extended cold ischemic storage before transplantation.