High frequency of identical clonal immunoglobulin DNA in pre-treatment tumor and plasma from untreated patients with HIV-associated lymphoma: prospective multicenter trial of the AIDS malignancies consortium (AMC 064). Issue 12 (2nd December 2017)

• Record Type: Journal Article
• Authors: Wagner-Johnston, Nina D.; Lensing, Shelly; Noy, Ariela; Ratner, Lee; Henry, David; Lee, Jeannette Y.; Silver, Sylvia; Faham, Malek; Ambinder, Richard F.
• Is Part Of: Leukemia & lymphoma. Volume 58:Issue 12(2017)
• Page Start: 2939
• Page End: 2942
• Publication Date: 2017-12-02
• Subjects: Lymphoma -- clones -- Immunoglobulin -- DNA -- HIV; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2017.1317095
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: Patients with HIV are at increased risk for developing B-cell lymphomas likely due in part to chronic antigen stimulation leading to clonal immunoglobulin (Ig) gene rearrangements. Next-generation sequencing (NGS)-based identification of circulating Ig clonotypes has not been well-characterized in HIV-related lymphomas. The AIDS Malignancies Consortium (AMC) enrolled 51 untreated patients with HIV-related B-cell lymphomas and analyzed paired tumor/plasma specimens for Ig clonotypes using an NGS approach (AMC064, NCT00981097). Lymphoma-specific clonotypes (>5% frequency) were identified in 83% (33/40) of tumor specimens. Results from paired tumor/plasma specimens showed identical circulating clonotypes in the plasma from 97% (32/33) of patients. High International Prognostic Index (IPI) scores of 3–4 among patients with B-cell lymphoma correlated with higher lymphoma molecules/million diploid genomes in the plasma compared with lower IPI scores of 0–2, median 77335 vs. 6876, p = .005. Further studies are merited to determine whether plasma clonal Ig DNA is prognostic in HIV-related lymphomas.