Cytotoxic T cells in chronic idiopathic neutropenia express restricted antigen receptors. Issue 12 (2nd December 2017)

• Record Type: Journal Article
• Authors: Mastrodemou, Semeli; Stalika, Evangelia; Vardi, Anna; Gemenetzi, Katerina; Spanoudakis, Michalis; Karypidou, Maria; Mavroudi, Irene; Hadzidimitriou, Anastasia; Stavropoulos-Giokas, Catherine; Papadaki, Helen A.; Stamatopoulos, Kostas
• Is Part Of: Leukemia & lymphoma. Volume 58:Issue 12(2017)
• Page Start: 2926
• Page End: 2933
• Publication Date: 2017-12-02
• Subjects: Chronic idiopathic neutropenia -- T cell receptor -- antigen selection; Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.1080/10428194.2017.1324154
• Languages: English
• ISSNs: 1042-8194

Abstract

Abstract: Chronic idiopathic neutropenia (CIN) is an acquired disorder of granulopoiesis characterized by female predominance and mostly uncomplicated course. Crucial to CIN pathophysiology is the presence of activated T lymphocytes with myelosuppressive properties in both peripheral blood (PB) and bone marrow (BM). We systematically profiled the T cell receptor beta chain (TRB) gene repertoire in CD8 + cells of 34 CIN patients through subcloning/Sanger sequencing analysis of TRBV–TRBD–TRBJ gene rearrangements. Remarkable repertoire skewing and oligoclonality were observed, along with shared clonotypes between different patients, alluding to antigen selection. Cross-comparison of our sequence dataset with public TRB sequence databases revealed that CIN may rarely share common immunogenetic features with other entities, however, the CIN TRB repertoire is largely disease-biased. Overall, these findings suggest that CIN may be driven by long-term exposure to a restricted set of specific CIN-associated antigens.