An encoded viral micropatch for multiplex cell-based assays through localized gene delivery. Issue 14 (30th May 2017)

• Record Type: Journal Article
• Authors: Han, Sangkwon; Bae, Hyung Jong; Kim, Su Deok; Park, Wook; Kwon, Sunghoon
• Is Part Of: Lab on a chip. Volume 17:Issue 14(2017)
• Page Start: 2435
• Page End: 2442
• Publication Date: 2017-05-30
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c7lc00372b
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : An encoded viral micropatch is presented for localized target gene expression for high-throughput, high-content cell-based assays. Abstract : The increasing number of potential drug targets and compounds has led to the development of high-throughput cell-based assays. Simultaneous processing of multiple targets in the same experiment based on localized target gene expression is a very efficient strategy for this purpose. To address this need, we present an adenoviral vector-immobilized microparticle with two-dimensional (2D) shape-encoding properties that allows localized patch-like gene delivery to monolayer-cultured cells. This format conveniently achieves multiplexed gene delivery compatible with both high-throughput cellular assays and fluorescence high-content imaging instruments. A multiplex G protein-coupled receptor (GPCR) internalization assay was developed to demonstrate the compatibility of this system with high-throughput image-based cellular assays.