A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis. Issue 57 (26th June 2017)

• Record Type: Journal Article
• Authors: Lou, Fei; Yang, Jie; Wu, Si; Perrett, Sarah
• Is Part Of: Chemical communications. Volume 53:Issue 57(2017)
• Page Start: 7986
• Page End: 7989
• Publication Date: 2017-06-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc03032k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Site-specific incorporation of donor–acceptor pair into a dimeric protein for single-molecule FRET analysis is realized based on a co-expression strategy. Abstract : A generic co-expression strategy for site-specific incorporation of a single donor–acceptor dye pair into any position in a dimeric protein, allowing single molecule FRET study of proteins previously inaccessible to this technique, such as the intrinsically disordered prion N-domain of Ure2 in the context of its globular dimeric C-domain.