Gold nanoparticles stabilized by cationic carbosilane dendrons: synthesis and biological properties. Issue 27 (16th January 2017)

• Record Type: Journal Article
• Authors: Peña-González, Cornelia E.; Pedziwiatr-Werbicka, Elzbieta; Shcharbin, Dzmitry; Guerrero-Beltrán, Carlos; Abashkin, Viktar; Loznikova, Svetlana; Jiménez, José L.; Muñoz-Fernández, M. Ángeles; Bryszewska, Maria; Gómez, Rafael; Sánchez-Nieves, Javier; de la Mata, F. Javier
• Is Part Of: Dalton transactions. Volume 46:Issue 27(2017)
• Page Start: 8736
• Page End: 8745
• Publication Date: 2017-01-16
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c6dt03791g
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : Water soluble gold nanoparticles have been isolated with pendant cationic carbosilane dendrons and their biological properties have been evaluated. Abstract : Gold nanoparticles (AuNPs) and polycationic macromolecules are used as gene carriers. Their behaviour is dependent on several factors, such as the size and type of the framework, charge, etc . We have combined both types of systems and prepared AuNPs covered with cationic carbosilane dendrons with the aim to evaluate their biocompatibility. Water soluble dendronized cationic AuNPs were prepared following a straightforward procedure from dendrons, a gold precursor and a reducing agent in water and were characterized by 1 H NMR, transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), ultraviolet spectroscopy (UV), and zeta potential (ZP). The biological properties of dendrons and AuNPs were determined by hemolysis, platelet aggregation and lymphocyte proliferation. These assays reflect modification of dendron properties when covering nanoparticles. For dendrons, hemolysis and platelet aggregation are generation dependent whilst, for AuNPs these properties are related to the bigger size of NPs. On the other hand, none of the systems induced lymphocyte proliferation. Selected cationic dendrons and AuNPs were chosen for gene delivery experiments employing a small interference RNA (siRNA Nef) against human immunodeficiency virus (HIV).