Prevalence and molecular epidemiology of Clostridium difficile infection in Indonesia. (July 2017)

• Record Type: Journal Article
• Authors: Collins, D.A.; Gasem, M.H.; Habibie, T.H.; Arinton, I.G.; Hendriyanto, P.; Hartana, A.P.; Riley, T.V.
• Is Part Of: New microbes and new infections. Volume 18(2017)
• Page Start: 34
• Page End: 37
• Publication Date: 2017-07
• Subjects: Clostridium difficile -- epidemiology -- Indonesia -- molecular epidemiology -- prevalence; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; Microbiology -- Periodicals; Communicable Diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1016/j.nmni.2017.04.006
• Languages: English
• ISSNs: 2052-2975

Abstract

Abstract: Clostridium difficile has not been studied in detail in Asia, particularly Southeast Asia. We thus performed a prevalence study across four hospitals in Central Java province, Indonesia. Stool samples were collected from patients with diarrhoea and tested by enzyme immunoassay for glutamate dehydrogenase (GDH) and toxin A/B (C DIFF QUIK CHEK COMPLETE, TechLab). Specimens were cultured and molecular typing was performed. In total, 340 samples were tested, of which 70 (20.6%) were GDH positive, with toxin detected in 19 (5.6%). Toxigenic C. difficile was isolated from 37 specimens (10.9%), while a further 36 (10.6%) nontoxigenic isolates were identified. The most common strain was ribotype 017 (24.3% of 74 isolates), followed by nontoxigenic types QX 224 (9.5%), and QX 238 and QX 108 (both 8.1%). The high prevalence of C. difficile highlights a need for ongoing surveillance of C. difficile infection in Indonesia.