Effect of 21-day head down bed rest on urine proteins related to endothelium: Correlations with changes in carbohydrate metabolism. (August 2017)

• Record Type: Journal Article
• Authors: Kashirina, D.; Pastushkova, L.; Custaud, M.A.; Dobrokhotov, I.; Brzhozovsky, A.; Navasiolava, N.; Nosovsky, A.; Kononikhin, A.; Nikolaev, E.; Larina, I.
• Is Part Of: Acta astronautica. Volume 137(2017)
• Page Start: 122
• Page End: 127
• Publication Date: 2017-08
• Subjects: Urine chromatography-mass spectrometry+ -- Physical inactivity -- Head-down bed rest -- Cardiovascular system -- Endothelial dysfunction; Astronautics -- Periodicals; Outer space -- Exploration -- Periodicals; Astronautics; Periodicals; 629.405
• DOI: 10.1016/j.actaastro.2017.04.020
• Languages: English
• ISSNs: 0094-5765

Abstract

Abstract: We performed liquid chromatography-mass spectrometric study of the urine proteome in 8 healthy volunteers aged between 20 and 44 y.o. who have completed 21-day head-down bed rest. ANDSystem software which builds associative networks was used to identify the urinary proteins functionally related to the endothelium. We identified 7 endothelium-related biological processes, directly linked to 13 urine proteins. We performed manual annotation of the proteins which were the most important in terms of endothelial functions. Analysis of the correlations with biochemical variables revealed a positive correlation between fasting blood glucose and the following urine proteins: albumin, CD44 antigen, endothelial protein C receptor, mucin-1, osteopontin, receptor tyrosine kinase. As well, we found a positive correlation between HOMA-insulin resistance index and the following urine proteins: endothelial protein C receptor and syndecan-4. These results might suggest the involvement of above-mentioned proteins in glucose metabolism and their participation in the response to changes in blood glucose level. Highlights: We identified 7 endothelium-related biological processes, linked to 13 urine proteins. Correlation between fasting glucose and ALBU, CD44, EPCR, UFO, MUC1, OSTP revealed. We performed manual annotation of endothelium-related proteins.