Sweat allergy: Extrinsic or intrinsic?. Issue 1 (July 2017)

• Record Type: Journal Article
• Authors: Hiragun, Takaaki; Hiragun, Makiko; Ishii, Kaori; Kan, Takanobu; Hide, Michihiro
• Is Part Of: Journal of dermatological science. Volume 87:Issue 1(2017:Jul.)
• Page Start: 3
• Page End: 9
• Publication Date: 2017-07
• Subjects: sweat -- atopic dermatitis -- cholinergic urticaria -- Malassezia globosa -- MGL_1304; AD atopic dermatitis -- CholU cholinergic urticari; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2017.03.002
• Languages: English
• ISSNs: 0923-1811

Abstract

Abstract: Sweat is an exacerbation factor in atopic dermatitis (AD) in all age groups. A body core temperature elevation with sweating triggers cholinergic urticaria (CholU). We recently reported that AD symptoms are improved by tannic acid-containing spray, which suppresses the basophil histamine release induced by semi-purified sweat antigen in vitro, and by showering, which removes antigens in sweat from the skin surface. Sweat contains small amount of proteins including proteases, protease inhibitors, and anti-microbial peptides. We finally identified MGL_1304 secreted by Malassezia ( M. ) globosa as a major histamine – releasing antigen in human sweat. MGL_1304 is a 17-kDa protein in sweat that elicits almost the highest histamine – release activity from basophils of patients with AD and CholU among antigens derived from Malassezia species. Moreover, serum levels of anti-MGL_1304 IgE were significantly higher in patients with AD and CholU than in normal controls. The recombinant protein produced by Pichia pastoris possessed comparable allergenicity to native MGL_1304. We found a monoclonal IgE antibody against MGL_1304 which did not elicit histamine release from sensitized mast cells. Desensitization therapy using autologous sweat, or MGL_1304 purified from culture of M. globosa or its cognates might be beneficial for patients with intractable CholU due to sweat allergy.