Metabolomics of fescue toxicosis in grazing beef steers. (July 2017)

• Record Type: Journal Article
• Authors: Mote, Ryan S.; Hill, Nicholas S.; Uppal, Karan; Tran, ViLinh T.; Jones, Dean P.; Filipov, Nikolay M.
• Is Part Of: Food and chemical toxicology. Volume 105(2017)
• Page Start: 285
• Page End: 299
• Publication Date: 2017-07
• Subjects: 12-HETE 12-hydroxyeicosatetreanoic acid -- 5HT serotonin -- 5HT1B serotonin 1B receptor -- 5HT1D serotonin 1D receptor -- 5HT2A serotonin 2A receptor -- AA-5-HT arachidonyl serotonin -- AE anion-exchange liquid chromatography -- AFMK N(1)-acetyl-N(2)-formyl-5-methoxykynuramine -- apLCMS adaptive processing liquid chromatography mass spectrometry -- BW body weight -- C18 reverse phase liquid chromatography -- D2 dopamine-2 receptor -- DA dopamine -- DHBA 3, 4-dihydroxybenzylamine -- DOPAC 3, 4-dihydroxyphenyl acetic acid -- E epinephrine -- ELISA enzyme linked immunosorbent assay -- EPI epinine -- FAAH fatty acid amide hydrolase -- FT fescue toxicosis -- FT-ICR Fourier transform ion cyclotron resonance -- HMDB Human Metabolome Database -- HPLC-ECD high performance liquid chromatography with electrochemical detection -- HRM high-resolution metabolomics -- HVA homovanillic acid -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LC liquid chromatography -- l-DOPA 3, 4-dihydroxyphenylalanine -- Lipid MAPS Lipid Metabolites and Pathways Strategy database -- LTQ linear triple quadrupole -- m/z mass to charge ratio -- Metlin Scripps Center for Metabolomics Metlin batch processing software -- MS mass spectrometry -- MS/MS tandem mass spectrometry -- NE norepinephrine -- NT non-toxic -- PAF platelet activating factor -- PCA principal component analysis -- PLS-DA partial least squares discriminant analysis -- T temperature -- THI temperature humidity index -- VFA volatile fatty acid; Fescue toxicosis -- Epichloë coenophiala -- Ergot alkaloids -- Grazing beef cattle -- Untargeted metabolomics; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.04.020
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Fescue toxicosis (FT) results from consumption of tall fescue ( Lolium arundinaceum ) infected with an endophyte ( Epichloë coenophiala ) that produces ergot alkaloids (EA), which are considered key etiological agents of FT. Decreased weight gains, hormonal imbalance, circulating cholesterol disruption, and decreased volatile fatty acid absorption suggest toxic (E+) fescue-induced metabolic perturbations. Employing untargeted high-resolution metabolomics (HRM) to analyze E+ grazing-induced plasma and urine metabolome changes, fescue-naïve Angus steers were placed on E+ or non-toxic (Max-Q) fescue pastures and plasma and urine were sampled before, 1, 2, 14, and 28 days after pasture assignment. Plasma and urine catecholamines and urinary EA concentrations were also measured. In E+ steers, urinary EA appeared early and peaked at 14 days. 13, 090 urinary and 20, 908 plasma HRM features were detected; the most significant effects were observed earlier (2 days) in the urine and later (≥14 days) in the plasma. Alongside EA metabolite detection, tryptophan and lipid metabolism disruption were among the main consequences of E+ consumption. The E+ grazing-associated metabolic pathways and signatures described herein may accelerate development of novel early FT detection and treatment strategies. Highlights: We applied high-resolution metabolomics (HRM) to plasma and urine of steers grazing toxic fescue. More than 13, 000 and almost 21, 000 unique metabolite features were detected in, respectively, the urine and plasma. The largest number of putative ergots alkaloids appeared after 2 or 14 days of grazing in, respectively, urine and plasma. Tryptophan and glycerophospholipid metabolism was perturbed by toxic fescue grazing. Novel plasma and urine metabolites, potential biomarkers of ergot alkaloid exposure and/or effect, were identified.