Large‐scale synthesis of tert‐butyl (3R, 5S)‐6‐chloro‐3, 5‐dihydroxyhexanoate by a stereoselective carbonyl reductase with high substrate concentration and product yield. (24th March 2017)

• Record Type: Journal Article
• Authors: Liu, Zhi‐Qiang; Hu, Zhong‐Liang; Zhang, Xiao‐Jian; Tang, Xiao‐Ling; Cheng, Feng; Xue, Ya‐Ping; Wang, Ya‐Jun; Wu, Lin; Yao, Dan‐Kai; Zhou, Yi‐Teng; Zheng, Yu‐Guo
• Is Part Of: Biotechnology progress. Volume 33:Number 3(2017)
• Page Start: 612
• Page End: 620
• Publication Date: 2017-03-24
• Subjects: carbonyl reductase -- tert‐butyl (S)‐6‐chloro‐5‐hydroxy‐3‐oxohexanoate -- tert‐butyl (3R, 5S)‐6‐chloro‐3, 5‐dihydroxyhexanoate -- asymmetric reduction -- space‐time yield; Biotechnology -- Periodicals; Food industry and trade -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/btpr.2460
• Languages: English
• ISSNs: 8756-7938

Abstract

Abstract : To biosynthesize the (3R, 5S)‐CDHH in an industrial scale, a newly synthesized stereoselective short chain carbonyl reductase (SCR) was successfully cloned and expressed in Escherichia coli. The fermentation of recombinant E. coli harboring SCR was carried out in 500 L and 5000 L fermenters, with biomass and specific activity of 9.7 g DCW/L, 15749.95 U/g DCW, and 10.97 g DCW/L, 19210.12 U/g DCW, respectively. The recombinant SCR was successfully applied for efficient production of (3R, 5S)‐CDHH. The scale‐up synthesis of (3R, 5S)‐CDHH was performed in 5000 L bioreactor with 400 g/L of (S)‐CHOH at 30°C, resulting in a space‐time yield of 13.7 mM/h/g DCW, which was the highest ever reported. After isolation and purification, the yield and d.e. of (3R, 5S)‐CDHH reached 97.5% and 99.5%, respectively. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:612–620, 2017