Heavily Boron Doped Diamond Electrodes for Ultra Sensitive Determination of Ciprofloxacin in Human Urine. Issue 6 (29th March 2017)

• Record Type: Journal Article
• Authors: Radičová, Monika; Behúl, Miroslav; Marton, Marián; Vojs, Marian; Bodor, Róbert; Redhammer, Robert; Vojs Staňová, Andrea
• Is Part Of: Electroanalysis. Volume 29:Issue 6(2017)
• Page Start: 1612
• Page End: 1617
• Publication Date: 2017-03-29
• Subjects: Antibiotics -- Boron doped diamond electrode -- Ciprofloxacin -- Human urine -- Voltammetry; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201600769
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Ciprofloxacin is one of the most potent fluoroquinolone antibiotics in medical treatment with a widely effective antibacterial activity. Aim of the presented paper was to use boron doped diamond electrodes for a sensitive, simple and reliable voltammetric determination of ciprofloxacin in human urine samples. Prior to the electrochemical analyses, an optimal boron doping level was determined in order to achieve the highest sensitivity. A set of boron doped diamond electrodes with the doping level in the range from 0 to 20 000 ppm B/C was used for this purpose. Electrochemical behavior of ciprofloxacin was investigated using cyclic voltammetry in an ammonium acetate buffer (pH 5), where ciprofloxacin provided a well‐defined irreversible oxidation peak at a potential of + 1.15 V. Under optimal experimental conditions, the calibration curve obtained by square‐wave voltammetry was linear in a concentration range from 0.15 to 2.11 μmol/L (R 2 =0.9974). A very low limit of detection (0.05 μmol/L) was obtained for the BDD electrode with the highest doping level. The developed square wave method was successfully applied to the determination of ciprofloxacin in human urine samples with a very good recovery (from 97 to 102%). Abstract :