Development and Optimization of an Ion‐selective Electrode for Serotonin Detection. Issue 6 (3rd April 2017)

• Record Type: Journal Article
• Authors: Bliem, Christina; Fruhmann, Philipp; Stoica, Anca‐Iulia; Kleber, Christoph
• Is Part Of: Electroanalysis. Volume 29:Issue 6(2017)
• Page Start: 1635
• Page End: 1642
• Publication Date: 2017-04-03
• Subjects: Electrodes -- Neurotransmitters -- Ionophores -- Carborane -- Polymeric membrane -- Plasticizer; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201600782
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Our studies are focused on the development of novel potentiometric sensors for the quantification of the neurotransmitter serotonin. Therefore, ion‐selective electrodes based on plasticized PVC membranes are applied. The electroactive part of the membrane consists of an ion pair complex formed between the protonated analyte and a carborane anion [Co(1, 2‐C2 B9 H11 )2 ] − . The analytical performance of the electrode was studied regarding sensitivity, concentration range, limit of detection and potential stability. The ion‐selective electrodes were optimized with respect to the material of the transducing element, as well as the membrane thickness and its composition. Stable, all solid state ISEs could be developed, using the non‐polar plasticizer NPOE and a graphite rod with high surface area as transducing element. We thus achieved a near Nernstian response over three decades of concentration (2.25⋅10 ‐5 ‐1.00⋅10 ‐2 M) and a limit of detection in the μ‐molar range for the optimized electrodes. The electrodes could successfully be miniaturized using carbon based screen printed electrodes. Abstract :