Urinary Tamm‐Horsfall protein, albumin, vitamin D‐binding protein, and retinol‐binding protein as early biomarkers of chronic kidney disease in dogs. Issue 11 (2nd June 2017)

• Record Type: Journal Article
• Authors: Chacar, Fernanda; Kogika, Márcia; Sanches, Talita R.; Caragelasco, Douglas; Martorelli, Cínthia; Rodrigues, Camila; Capcha, Jose Manuel C.; Chew, Dennis; Andrade, Lúcia
• Is Part Of: Physiological reports. Volume 5:Issue 11(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-06-02
• Subjects: Chronic kidney disease -- dogs -- retinol‐binding protein -- Tamm‐Horsfall protein -- vitamin D‐binding protein; Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.13262
• Languages: English
• ISSNs: 2051-817X

Abstract

Abstract: Proteinuria is a marker and mediator of chronic kidney disease (CKD). In clinical practice, the urinary protein‐to‐creatinine ratio (UP/C) is of limited usefulness, because it indicates only the magnitude of proteinuria and not the origin of the loss (glomerular or tubular). The complete assessment of proteinuria includes quantitative and qualitative evaluations, both of which are required in order to optimize the therapy. In addition to measuring the UP/C, we performed SDS‐PAGE and western blotting to determine the expression of albumin, vitamin D‐binding protein (VDBP), retinol‐binding protein (RBP), and Tamm‐Horsfall protein (THP) in urine samples of 49 dogs: healthy (control) dogs ( n = 9); and dogs with CKD ( n = 40), stratified by stage. In the dogs with stage 3 or 4 CKD, there was a predominance of tubular proteins. Neither VDBP nor RBP was observed in the urine of the control dogs. Among the dogs with stage 1 or 2 CKD, VDBP and RBP were detected in those without proteinuria or with borderline proteinuria. The expression of urinary albumin was significantly higher in the stage 4 group than in any other group ( P ≤ 0.01). In the stage 4 group, urinary THP was either undetectable or lower than in the control group ( P ≤ 0.01). In conclusion, urinary VDBP and RBP might act as early markers of kidney injury, and a decrease in urinary THP could be an indicator of CKD progression. Abstract : Urinary Vitamin D‐binding protein and Retinol binding‐protein may act as early biomarkers of CKD in dogs; Tamm‐Horsfall serve as a marker of CKD progression in dogs.