Bud detachment in hydra requires activation of fibroblast growth factor receptor and a Rho–ROCK–myosin II signaling pathway to ensure formation of a basal constriction. Issue 7 (22nd May 2017)

• Record Type: Journal Article
• Authors: Holz, Oliver; Apel, David; Steinmetz, Patrick; Lange, Ellen; Hopfenmüller, Simon; Ohler, Kerstin; Sudhop, Stefanie; Hassel, Monika
• Is Part Of: Developmental dynamics. Volume 246:Issue 7(2017)
• Page Start: 502
• Page End: 516
• Publication Date: 2017-05-22
• Subjects: receptor tyrosine kinase -- actin -- Rhosin -- myosin; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24508
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract : Background : Hydra propagates asexually by exporting tissue into a bud, which detaches 4 days later as a fully differentiated young polyp. Prerequisite for detachment is activation of fibroblast growth factor receptor (FGFR) signaling. The mechanism which enables constriction and tissue separation within the monolayered ecto‐ and endodermal epithelia is unknown.Results: Histological sections and staining of F‐actin by phalloidin revealed conspicuous cell shape changes at the bud detachment site indicating a localized generation of mechanical forces and the potential enhancement of secretory functions in ectodermal cells. By gene expression analysis and pharmacological inhibition, we identified a candidate signaling pathway through Rho, ROCK, and myosin II, which controls bud base constriction and rearrangement of the actin cytoskeleton. Specific regional myosin phosphorylation suggests a crucial role of ectodermal cells at the detachment site. Inhibition of FGFR, Rho, ROCK, or myosin II kinase activity is permissive for budding, but represses myosin phosphorylation, rearrangement of F‐actin and constriction. The young polyp remains permanently connected to the parent by a broad tissue bridge.Conclusions: Our data suggest an essential role of FGFR and a Rho‐ROCK‐myosin II pathway in the control of cell shape changes required for bud detachment. Developmental Dynamics 246:502–516, 2017 . © 2017 The Authors Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists Key Findings: Hydra bud detachment involves the separation of two intact epithelia without cell death. Remarkable cell shape changes and multicellular rosettes at the bud base indicate functional specification and strong mechanical forces. mRNA colocalization, phospho‐myosin analysis and similar phenotypes obtained by pharmacological inhibition suggest a tight correlation between FGFR and a Rho‐ROCK‐Myosin II candidate signaling pathway.