SDF-1/CXCL12 and CXCR4 gene variants, and elevated serum SDF-1 levels are associated with preeclampsia. (3rd April 2017)

• Record Type: Journal Article
• Authors: Karakus, Savas; Bagci, Binnur; Bagci, Gokhan; Sancakdar, Enver; Yildiz, Caglar; Akkar, Ozlem; Cetin, Ali
• Is Part Of: Hypertension in pregnancy. Volume 36:Number 2(2017)
• Page Start: 124
• Page End: 130
• Publication Date: 2017-04-03
• Subjects: CXCL12 gene -- CXCR4 gene -- SDF-1 -- polymorphism -- preeclampsia; Hypertension in pregnancy -- Periodicals; Pregnancy -- Complications -- Periodicals; 618.3261
• DOI: 10.1080/10641955.2016.1250904
• Languages: English
• ISSNs: 1064-1955

Abstract

ABSTRACT: Objective : We aimed to compare the frequencies of stromal cell-derived factor-1 (SDF-1) 3′A and CXCR4 single-nucleotide polymorphisms (SNPs) and serum SDF-1 levels in patients with preeclampsia (PE). Methods : In total, 89 women with PE and 89 control women were included in the study. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism method. Enzyme-linked immunosorbent assay method was used to measure serum SDF-1 level. Results : For SDF-1 3′A SNP, the frequency of GA genotype, total number of GA and AA genotypes, and the A allele frequency was higher in PE patients than controls ( p = 0.04, 0.023, and 0.029, respectively). For CXCR4 SNP, the frequency of CT genotype, total number of CT and TT genotypes, and the T allele frequency were higher in PE patients than controls ( p = 0.04, 0.006, and 0.005, respectively). SDF-1 serum level was detected higher in preeclamptic women compared with controls ( p = 0.001). In PE patients, there was no significant association between serum SDF-1 levels and genotypes of SDF-1 3′A SNP. SDF-1 level was significantly higher in patients bearing CXCR4 CT genotype than CC genotype ( p = 0.001). Furthermore, SDF-1 levels in patients bearing CT+TT genotype were found higher than that of patients with CC genotypes ( p = 0.001). Conclusion : Results of our study suggest that SDF-1 3′A and CXCR4 polymorphisms and elevated serum SDF-1 levels may have a role in the development of PE.